EGR4 ELISA Kit (Mouse)

• Reproducibility:

  Inter-Assay CV

• Gene Aliases:

  NGFI, pAT1, NGF1-, NGFIC, NGF1-C, NGFI-C, pAT133

• Gene ID:

  13656

• Accession Number:

  NP_065621

• Reactivity:

  Mouse

• Target:

  Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG) . Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Egr4 ELISA Kit (Mouse) (OKEI00444) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Egr4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Egr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Egr4

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Biological Fluids

• Detection Range:

  0.078 - 5 ng/mL

• Sensitivity:

  46.9 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  EGR4

• Protein Name:

  Early growth response protein 4

• Gene Name URL:

  EGR4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_020596