PSIP1 ELISA Kit (Rat)

• Gene Name:

  PC4 and SFRS1 interacting protein 1

• Gene Aliases:

  Ledgf; lens epithelium-derived growth factor; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; Psip2; SBP75; supercoiled DNA binding protein 75.

• Gene ID:

  313323

• Accession Number:

  NP_786941.1

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  PC4 and SFRS1-interacting protein

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Psip1 ELISA Kit (Rat) (OKEH04199) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Psip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psip1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

• Detection Range:

  0.156-10ng/ml

• Sensitivity:

  0.078 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  60 kDa

• Specificity:

  Natural and recombinant Rat PC4 and SFRS1-interacting protein

• Protein Length:

  528

• NCBI Gene Symbol:

  Psip1

• Protein Name:

  Q812D1

• Gene Name URL:

  Psip1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_175765.2