Thromboxane A2 ELISA Kit

• Gene Aliases:

  TXA2, Thromboxane A2, TXA2

• Reactivity:

  All Species

• Target:

  Thromboxane A2 (TXA2) is a thromboxane. It is produced by activated platelets and has prothrombotic properties: it stimulates activation of new platelets as well as increases platelet aggregation. This is achieved by mediating expression of the glycoprotein complex GP IIb/IIIa in the cell membrane of platelets. Circulating fibrinogen binds these receptors on adjacent platelets, further strengthening the clot. TXA2 is generated from prostaglandin H2 by thromboxane-A synthase. Aspirin irreversibly inhibits platelet cyclooxygenase 1 preventing the formation of prostaglandin H2, and therefore thromboxane A2.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology Thromboxane A2 ELISA Kit (OKEH02590) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Thromboxane A2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thromboxane A2 and incubated. The Thromboxane A2 found in the sample or standards competes with the biotinylated Thromboxane A2 for limited binding sites on the immobilized anti-Thromboxane A2 antibody. Excess unbound biotinylated Thromboxane A2 and sample or standard Thromboxane A2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thromboxane A2 captured in the well and inversely proportional to the amount of Thromboxane A2 which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

• Detection Range:

  3.12-200pg/mL

• Recovery:

  <= 6.6%

• Sensitivity:

  1.25 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant General Thromboxane A2

• Protein Name:

  Thromboxane A2

• CAS Number:

  7732-18-5