DDR1 ELISA Kit (Human)

• Gene Name:

  Discoidin domain receptor tyrosine kinase 1

• Gene Aliases:

  CAK; CD167; CD167 antigen-like family member A; cell adhesion kinase; DDR; Discoidin receptor tyrosine kinase; EDDR1; epithelial discoidin domain-containing receptor 1; HGK2; mammary carcinoma kinase 10; MCK10; NEP; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; NTRK4; Protein-tyrosine kinase 3A; protein-tyrosine kinase RTK-6; PTK3; PTK3A; PTK3A protein tyrosine kinase 3A; RTK6; TRK E; TRKE; tyrosine kinase DDR; tyrosine-protein kinase CAK.

• Gene ID:

  780

• Accession Number:

  NP_001189450.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Epithelial discoidin domain-containing receptor 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology DDR1 ELISA Kit (Human) (OKEH02503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDR1 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%

• Sensitivity:

  0.056 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  101 kDa

• Specificity:

  Natural and recombinant Human Epithelial discoidin domain-containing receptor 1

• Protein Length:

  913

• NCBI Gene Symbol:

  DDR1

• Protein Name:

  Q08345

• Gene Name URL:

  DDR1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001202521.1