CTGF Chemi-Luminescent ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Cellular communication network factor 2

• Gene Aliases:

  Ccn; CCN family member 2; cellular communication network factor 1; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24) ; Ctgf; fibroblast inducible secreated protein; fibroblast inducible secreted protein; Fis; fisp-12; Fisp12; Hcs2; Hcs24; hypertrophic chondrocyte-specific gene product 24; hypertrophic chondrocyte-specific protein 24; Protein FISP-12.

• Gene ID:

  14219

• Accession Number:

  NP_034347

• Reactivity:

  Mouse|Mus musculus

• Target:

  Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity) . Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity) . Enhances fibroblast growth factor-induced DNA synthesis (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  13.7-10,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 93-102 96 EDTA Plasma (n=5) 90-104 99 Heparin Plasma (n=5) 78-101 82 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-102 96 Serum (n=5) 93-102 96 EDTA Plasma (n=5) 90-104 99 EDTA Plasma (n=5) 90-104 99 Heparin Plasma (n=5) 78-101 82 Heparin Plasma (n=5) 78-101 82

• Sensitivity:

  0.156 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Ctgf Microplate 96 Wells (12 x 8 Well strips) Ctgf Lyophilized Standard 2 100X Ctgf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Ctgf Microplate 96 Wells (12 x 8 Well strips) Ctgf Microplate 96 Wells (12 x 8 Well strips) Ctgf Lyophilized Standard 2 Ctgf Lyophilized Standard 2 100X Ctgf Biotinylated Detector Anitbody 1 x 120 uL 100X Ctgf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Ccn2

• Protein Name:

  Connective tissue growth factor

• Gene Name URL:

  Ccn2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010217