NNMT ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Nicotinamide-N-Methyltransferase (NNMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Nicotinamide-N-Methyltransferase (NNMT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Nicotinamide N-methyltransferase

• Gene Aliases:

  Nicotinamide N-methyltransferase.

• Gene ID:

  18113

• Accession Number:

  NP_035054.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide-N-Methyltransferase (NNMT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide-N-Methyltransferase (NNMT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide-N-Methyltransferase (NNMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nicotinamide-N-Methyltransferase (NNMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 79-101 95 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 80-99 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-101 95 Serum (n=5) 79-101 95 EDTA Plasma (n=5) 95-103 99 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 80-99 91 Heparin Plasma (n=5) 80-99 91

• Sensitivity:

  0.055 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-NNMT Microplate 96 Wells (12 x 8 Well strips) NNMT Lyophilized Standard 2 100X Biotinylated NNMT Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-NNMT Microplate 96 Wells (12 x 8 Well strips) Anti-NNMT Microplate 96 Wells (12 x 8 Well strips) NNMT Lyophilized Standard 2 NNMT Lyophilized Standard 2 100X Biotinylated NNMT Detector Antibody 1 x 120 uL 100X Biotinylated NNMT Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Nicotinamide-N-Methyltransferase (NNMT) . No significant cross-reactivity or interference between Nicotinamide-N-Methyltransferase (NNMT) and analogues was observed.

• Protein Length:

  264

• NCBI Gene Symbol:

  Nnmt

• Protein Name:

  Nicotinamide N-methyltransferase

• Gene Name URL:

  Nnmt

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010924.3