CTSL ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cathepsin L (CTSL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cathepsin L (CTSL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Cathepsin L

• Gene Aliases:

  1190035F06Rik; Cat L; Cathepsin L; cathepsin L1; CatL; Ctsl1; fs; major excreted protein; ME; MEP; nkt; p39 cysteine proteinase; procathepsin L.

• Gene ID:

  13039

• Accession Number:

  NP_034114.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Important for the overall degradation of proteins in lysosomes.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin L (CTSL) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin L (CTSL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin L (CTSL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin L (CTSL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  62.5-4,000pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 96-105 102 EDTA Plasma (n=5) 85-98 90 Heparin Plasma (n=5) 81-98 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-105 102 Serum (n=5) 96-105 102 EDTA Plasma (n=5) 85-98 90 EDTA Plasma (n=5) 85-98 90 Heparin Plasma (n=5) 81-98 92 Heparin Plasma (n=5) 81-98 92

• Sensitivity:

  26.2 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-CTSL Microplate 96 Wells (12 x 8 Well strips) CTSL Lyophilized Standard 2 100X Biotinylated CTSL Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CTSL Microplate 96 Wells (12 x 8 Well strips) Anti-CTSL Microplate 96 Wells (12 x 8 Well strips) CTSL Lyophilized Standard 2 CTSL Lyophilized Standard 2 100X Biotinylated CTSL Detector Antibody 1 x 120 uL 100X Biotinylated CTSL Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Cathepsin L (CTSL) . No significant cross-reactivity or interference between Cathepsin L (CTSL) and analogues was observed.

• Protein Length:

  334

• NCBI Gene Symbol:

  Ctsl

• Protein Name:

  Cathepsin L1

• Gene Name URL:

  Ctsl

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009984.4