TNNI3 ELISA Kit (Rabbit)

• Reproducibility:

  Mean Inter-Assay Variation

• Reactivity:

  Rabbit

• Target:

  Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology TNNI3 ELISA Kit (Rabbit) (OKCA02208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNNI3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNNI3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TNNI3 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  47 pg/mL-3,000 pg/mL

• Recovery:

  Sample Type Mean (%) Range (%) Serum (n=5) 87 83-94 EDTA plasma (n=4) 89 85-97 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 87 83-94 Serum (n=5) 87 83-94 EDTA plasma (n=4) 89 85-97 EDTA plasma (n=4) 89 85-97

• Sensitivity:

  11.75 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  TNNI3

• Protein Name:

  Troponin I, cardiac muscle

• Gene Name URL:

  TNNI3

• CAS Number:

  7732-18-5