LDHA ELISA Kit (Rat)

• Reproducibility:

  Inter-Assay CV:

• Gene Name:

  L-lactate dehydrogenase A chain

• Gene Aliases:

  LDH-M

• Gene ID:

  100009107

• Accession Number:

  NP_001075746.1

• Reactivity:

  Rat

• Target:

  This protein is involved in step 1 of the subpathway that synthesizes (S) -lactate from pyruvate.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology LDHA ELISA Kit (Rat) (OKCA00980) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for LDHA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LDHA captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  39 mU/mL-2,500 mU/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 93 89-100 EDTA Plasma (n=4) 90 85-95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93 89-100 Serum (n=5) 93 89-100 EDTA Plasma (n=4) 90 85-95 EDTA Plasma (n=4) 90 85-95

• Sensitivity:

  9.77mU/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  LDHA

• Protein Name:

  L-lactate dehydrogenase A chain

• Gene Name URL:

  LDHA

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082277.1