Anti-NMDA Receptor, NR2A Subunit N-terminus

• Gene Aliases:

  NR2A, GluN2A, NMDAR2A

• Gene ID:

  24409

• Accession Number:

  NP_036705.3

• Reactivity:

  Mouse, Rat, Cow, Dog

• Immunogen:

  Peptide from the N-terminus of the NR2A subunit of rat NMDA receptor.

• Target:

  The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR) . Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996) . The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999) . The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification:

  Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Homology:

  Cow; Dog; Mouse; Rat

• Format:

  Liquid. 100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

• Reconstitution:

  Store at -20C; stable for at least 1 year

• Molecular Weight:

  ~180kDa

• Specificity:

  Specific for the ~180k NR2A subunit of the NMDA receptor.

• NCBI Gene Symbol:

  GRIN2A

• Host or Source:

  Rabbit

• Protein Name:

  Glutamate receptor ionotropic, NMDA 2A

• Gene Name URL:

  GRIN2A

• CAS Number:

  9007-83-4