Anti-DnaJ (E. coli) Antibody

• Gene Name:

  Chaperone protein DnaJ

• Gene Aliases:

  B0015; chaperone protein DnaJ; ECK0015; groP; grpC; Heat shock protein J; HSP40.

• Gene ID:

  944753

• Reactivity:

  Escherichia coli

• Immunogen:

  E. coli DnaJ.

• Target:

  Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunoprecipitation|Western blot

• Purification:

  Protein A affinity purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid.// PBS containing 50% glycerol and 0.09% sodium azide.

• Molecular Weight:

  ~41kDa

• NCBI Gene Symbol:

  DnaJ

• Host or Source:

  Rabbit

• Protein Name:

  Chaperone protein DnaJ

• Gene Name URL:

  DnaJ

• CAS Number:

  9007-83-4