HLA Class II (DR) Antibody

• Gene Name:

  Major histocompatibility complex, class II, DR alpha

• Gene Aliases:

  HLA-DRA1

• Gene ID:

  3122; 3125

• Accession Number:

  NP_061984.2

• Reactivity:

  Human

• Target:

  HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

• Clonality:

  Monoclonal

• Clone:

  B-F1

• Type:

  Monoclonal Antibody

• Applications:

  FC, IP

• Purification:

  Ion exchange chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 tests/2mL

• Format:

  Lyophilized

• Buffer:

  Phosphate-buffered saline with 1 % BSA and 0.09 % sodium azide

• Reconstitution:

  Stable at +2-8C for 12 months. For longer storage, freeze aliquots

• Molecular Weight:

  29 kDa

• Fragment:

  Mouse IgG1

• Specificity:

  Recognises a 28 kDa and 33 kDa protein

• NCBI Gene Symbol:

  HLA Class II

• Host or Source:

  Mouse

• Protein Name:

  HLA class II histocompatibility antigen, DR alpha chain, HLA class II histocompatibility antigen, DR beta 3 chain

• Gene Name URL:

  HLA Class II

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_019111.4