SETD7 Antibody - N-terminal region

• Gene Name:

  SET domain containing (lysine methyltransferase) 7

• Gene Aliases:

  KMT7, SET7, SET9, SET7/9

• Gene ID:

  80854

• Swiss Prot:

  Q8WTS6

• Accession Number:

  NP_085151

• Reactivity:

  Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish

• Immunogen:

  The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETD7

• Target:

  SETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

• Partner Proteins:

  KLF4; HIST3H3; SOX2; PRKAR1A; A1BG; TP53; NR2F2; STEAP3; SERTAD1; DPY30; MED1; MYOD1; E2F1; HIST1H3A; SMARCA4; H3F3A; STAT3; RELA; AR; UBC; DNMT1; PPP1R12A; SIRT1; TBP; TAF10; TAF7; TAF6; HIST1H4A; ESR1; HIST2H3C; HCFC1; L3MBTL1

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Sequence:

  MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGK

• Applications:

  WB

• Purification:

  Affinity purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Homology:

  Cow: 93%; Dog: 93%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Reconstitution:

  For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

• Molecular Weight:

  40kDa

• Protein Length:

  366

• NCBI Gene Symbol:

  SETD7

• Host or Source:

  Rabbit

• Protein Name:

  Histone-lysine N-methyltransferase SETD7

• Gene Name URL:

  SETD7

• CAS Number:

  9007-83-4