Universal Protease (established for quantitative determination for HtrA-, MMP-, ADAMTS-Protease among other, using HtrA1 as standard) ELISA (Activity Assay) Kit
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Catalog number30 516 103
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PricePlease ask
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Size1 Microplate/40 determinations
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ELISA categoryELISA (Activity Assay) Kit
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ELISA detection limits0.312 - 20 nM HtrA1
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ELISA sensitivity312 pM HtrA1
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ELISA s descriptionThe Universal Protease Activity ELISA measures activity of proteases like matrix metalloproteases (MMPs), A Disintegrin And Metalloproteinase with Thrombospondin Motifs (ADAMTS) and especially High temperature requirement factor A (HtrA). It consists of two modules: Protease module and the ELISA module.
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Research area interestsCancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
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Package form96 well microplate
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AntigenProtease
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Reacts withProtease
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Tested applicationsThe casein based assay measures activity of proteases. It is used to screen and characterize inhibitors.
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SpecificityProtease
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CrossreactivityN/A
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Preservative materialsIf applicable mentioned in the product data sheet
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Storage recommendations6 month
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Shipping and storage conditionsdry Ice and ice pack
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Long term storage-20 °C and 4 °C
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Assay principleProteolytic Reaction and Sandwich-ELISA
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Included in the standard packagemicroplate, buffers, antibody conjugate,standards
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Not included in the standard packageSpectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
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Samples to be analyzedbiological fluids, cell culture
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PubMed citationsSee the datasheet
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Technical datasheetContact us to receive the datasheet
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Representative figures
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WarningsFor Research Use only.
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Testbiotez supplies other types of Assays as 1. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Gene target
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Gene symbolADAMTS3, ADAMTS4, ADAMTS2, MMP14, MMP24, MMP28, MMP17, MMP15, MMP25, MMP16
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Short nameUniversal Protease (established for quantitative determination for HtrA-, MMP-, ADAMTS-Protease among other, using HtrA1 as standard) ELISA (Activity Assay) Kit
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TechniqueStandard, Assay, ELISA, assays, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. standards
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HostAssay, antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative nameUniversal Protease (established to measure volumetric measurement to measure HtrA-, MMP-, ADAMTS-Protease among other, using HtrA serine peptidase 1 as reference) Enzyme-linked immunosorbent assay (Activity test) reagent
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Alternative techniquestandards, arrays, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameADAM metallopeptidase with thrombospondin type 1 motif 3
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Synonyms gene name
- a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-04-15
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Entrez gene record
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Pubmed identfication
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Classification
- ADAM metallopeptidases with thrombospondin type 1 motif
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameADAM metallopeptidase with thrombospondin type 1 motif 4
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Synonyms gene name
- a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-04-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ADAM metallopeptidases with thrombospondin type 1 motif
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameADAM metallopeptidase with thrombospondin type 1 motif 2
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Synonyms gene name
- a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-04-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ADAM metallopeptidases with thrombospondin type 1 motif
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 14
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Synonyms gene name
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 24
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Synonyms gene name
- matrix metalloproteinase 24 (membrane-inserted)
- matrix metallopeptidase 24 (membrane-inserted)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 28
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Synonyms gene name
- matrix metalloproteinase 28
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 17
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Synonyms gene name
- matrix metalloproteinase 17 (membrane-inserted)
- matrix metallopeptidase 17 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 15
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Synonyms gene name
- matrix metalloproteinase 15 (membrane-inserted)
- matrix metallopeptidase 15 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 25
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 25
- matrix metallopeptidase-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-12-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 16
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 16 (membrane-inserted)
- chromosome 8 open reading frame 57
- matrix metallopeptidase 16 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data