Spoligotyping Kit with Primers and Controls

• Catalog number
  IM9701
• Price
  Please ask
• Size
  1 EA

• Availability
  Please contact Gentaur/Genprice to check the latest availability
• Ordering
  Current price is for Diagnostics labs. Special -16% discount for Research Institutes. For ordering please contact us at +32 16 58 90 45
• Technical file
  Please contact us to request the current datasheet or security datasheet
• Description
  Positive controls are often requested for publication of your result. Reference 1 can be used to spike a vast number of experiments, A primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand
• Test
  A rapid, polymerase chain reaction (PCR)-based method for genotyping strains of the Mycobacterium tuberculosis complex (MTB). Spoligotyping data can be represented in absolute terms (digitally), and the results can be readily shared among laboratories, thereby enabling the creation of large international databases. Since the spoligotype assay was standardized more than 10 yr. ago, tens of thousands of isolates have been analyzed, giving a global picture of MTB strain diversity. The GENTAUR method is highly reproducible and has been developed into a high-throughput assay for large molecular epidemiology projects. In the United States, spoligotyping is employed on nearly all newly identified culture-positive cases of tuberculosis as part of a national genotyping program. The strengths of this method include its low cost, its digital data results, the good correlation of its results with other genetics markers, its fair level of overall differentiation of strains, its high-throughput capacity, and its ability to provide species information. However, the method's weaknesses include the inability of spoligotyping to differentiate well within large strain families such as the Beijing family, the potential for convergent evolution of patterns, the limited success in improving the assay through expansion, and the difficulty in obtaining the specialized membranes and instrumentation

• Gene target
  Kit   with  
• Short name
  Spoligotyping Kit with Primers Controls
• Technique
  Spoligotyping, Controls, primers, control, primer, spoligotypings
• Alternative name
  Spoligotyping reagent including Primers and Controls
• Alternative technique
  mycobacteria, controls, pcr, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Amplified Fragment Length Polymorphism Analysis
• Concept
  Scope note: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.
• Tree numbers
  • E05.393.290.382
  • E05.393.620.500.324
• Qualifiers
  drug effects, radiation effects, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products