Sox2 Recombinant Protein

• Catalog number
  40-119
• Price
  Please ask
• Size
  0.005 mg, 0.025 mg

• About recombinant protein
  Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Recombinant human Sox2 is a 34.3 kDa protein containing 317 amino-acid residues.
• Other name of the recombinant
  ANOP3, MCOPS3, Transcription factor SOX-2
• Recombinant in
  E. coli
• SEQUENCE
  MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHM
• Keep at
  The lyophilized Sox2 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Sox2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Sox2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  28195386
• Accession nr
  NP_003097.1
• Pureity
  Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  6657
• Gene name
  SRY (sex determining region Y)-box 2
• Source
  Homo sapiens
• Uniprot
  P48431
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Sox2   Protein  
• Gene symbol
  SOX2
• Short name
  Sox2 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  SRY (sex determining region Y)-box 2 Rec. Protein
• Alternative technique
  rec
• Alternative to gene target
  SRY (sex determining region Y)-box 2, ANOP3 and MCOPS3, SOX2 and IDBG-66692 and ENSG00000181449 and 6657, protein heterodimerization activity, nuclei, Sox2 and IDBG-137912 and ENSMUSG00000074637 and 20674, SOX2 and IDBG-647279 and ENSBTAG00000011598 and 784383

Gene info

• Identity
  HGNC:11195
• Gene
  SOX2
• Long gene name
  SRY-box transcription factor 2
• Synonyms gene name
  • SRY (sex determining region Y)-box 2
  • SRY-box 2
• GenBank acession
  • BC013923
• Locus
  3q26.33
• Discovery year
  1993-11-30
• Entrez gene record
  6657
• Pubmed identfication
  • 7849401
• RefSeq identity
  • NM_003106
• Classification
  • SRY-box transcription factors
• VEGA ID
  OTTHUMG00000158222
• Locus Specific Databases
  • LOVD
  • LRG_719

Gene info

• Identity
  HGNC:20209
• Gene
  SOX2-OT
• Long gene name
  SOX2 overlapping transcript
• Synonyms gene
  • SOX2OT
• Synonyms gene name
  • SOX2 overlapping transcript (non-protein coding)
• Synonyms
  • DKFZp761J1324
  • NCRNA00043
• Synonyms name
  • non-protein coding RNA 43
• GenBank acession
  • AL157425
  • AK022826
• Locus
  3q26.33
• Discovery year
  2003-07-01
• Entrez gene record
  347689
• Pubmed identfication
  • 12612584
  • 19767420
  • 28960757
• RefSeq identity
  • NR_004053
• Classification
  • Overlapping transcripts
• VEGA ID
  OTTHUMG00000158186

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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