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Category
Primary Antibodies
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Long description
Atrazine is a herbicide against pre- and post-emergence broadleaf and grassy weeds. It is a very persistent compound and it has been banned in the EU since 2004. Its main toxic effect is as endocrine diruptor.
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Antibody come from
Serum of sheep immunised with BSA-atrazine conjugate, and purified using caprylic acid
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Other description
Each vial contains 0.5 ml of purified (caprylic acid) Sheep Polyclonal IgG
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Clone
Polyclonal
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Antigen antibody binding interaction
Sheep anti Atrazine Antibody
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Antibody is raised in
Sheep
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
Cross Reactivity Atrazine 100% Propazine 150% Simazine 4% Ametryn 2500% Prometryn 1700% Simetryn 280% Terbutylazine 2% Atrazine-desethyl 7% Atrazine desisopropyl 1% Atrazine-2-hydroxy 1% Cinosulfuron
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Antibody s specificity
Immunogen: BSA-atrazine conjugate, Target: Atrazine, CAS no.: 1912-24-9. The specificity of the antibody was determined by measuring the cross-reactivity with a range of compounds in ELISA.
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Research interest
Toxic Compounds
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Application
ELISA/Immuno-affinity-chromatography
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Antibody s suited for
Depending on conjugate: dilution 1/500 . (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1/500 from the delivered solution. Plates are coated with 400 ng/ml OVA-conjugated Atrazine. HRP-conjugated anti-sheep IgG as a tracer 1/8,000
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Storage
Store at 4 °C or aliquot and store at -20 °C for longer periods. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
