SCF Recombinant Protein

• Catalog number
  40-608
• Price
  Please ask
• Size
  0.01 mg

• About recombinant protein
  SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
• Other name of the recombinant
  SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
• Activity
  The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is = 2.0 ng/mL, corresponding to a specific activity of = 5 x 105 units/mg.
• Keep at
  The lyophilized SCF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted SCF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing SCF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4505175
• Accession nr
  NP_000890.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  4254
• Gene name
  KIT ligand
• Source
  Homo sapiens
• Uniprot
  P21583
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SCF   Protein  
• Gene symbol
  KITLG
• Short name
  SCF Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  SCF Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6343
• Gene
  KITLG
• Long gene name
  KIT ligand
• Synonyms gene
  • MGF
• Synonyms
  • SCF
  • SF
  • Kitl
  • KL-1
  • FPH2
  • SLF
  • DFNA69
• Synonyms name
  • mast cell growth factor
  • stem cell factor
  • steel factor
  • familial progressive hyperpigmentation 2
• GenBank acession
  • M59964
• Locus
  12q21.32
• Discovery year
  1991-06-04
• Entrez gene record
  4254
• Pubmed identfication
  • 2208279
  • 1707188
  • 19375057
  • 26522471
• RefSeq identity
  • NM_003994
• VEGA ID
  OTTHUMG00000169888
• Locus Specific Databases
  • LRG_1379

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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