Red Fluorescent Protein - R-PE (R-Phycoerythrin)
-
Catalog number6005-1
-
PricePlease ask
-
Size1 mg
-
-
SynonymsRFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
-
Alternative_namesRFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
-
DescriptionFluorescent protein which can be cross linked or conjugated to other molecules to make fluorescent probes.
-
RecombinantNo
-
SourceFebico’s proprietary red algae
-
Purity by SDS PAGEA566/A280 ≥ 5.5
-
AssayAbsorbance
-
PurityA566/A498 ≤1.5
-
Molecular Weight240 kDa
-
Storage Temp2-8°C
-
ShippingGel pack
-
Shelf Life60 months
-
AppearanceSuspension
-
Physical form descriptionR-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.
-
Reconstitution InstructionsBefore use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tubes) or desalted in columns (BioVision Cat # 6564-25) prior to use, to remove any remaining ammonium sulphate.
-
Background InformationR-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
Additional descriptionFluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
-
Conjugationred dye
-
Gene target
-
Gene symbolRPE, ETV3, ERF, RRAS, DAD1P1, HNRNPR, CLDN4, MC1R, CTNNA2, PTPRK
-
Short nameRed Protein - R-PE (R-Phycoerythrin)
-
LabelRed
-
Alternative nameRed Fluorescent Protein - R-peroxidase (R-Phycoerythrin)
-
Gene info
-
Identity
-
Gene
-
Long gene nameribulose-5-phosphate-3-epimerase
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameETS variant transcription factor 3
-
Synonyms gene name
- ets variant gene 3, ETS family transcriptional repressor
- ETS variant 3
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1994-12-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- ETS transcription factor family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameETS2 repressor factor
-
Synonyms gene name
- Ets2 repressor factor
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-07-17
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- ETS transcription factor family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRAS related
-
Synonyms gene name
- related RAS viral (r-ras) oncogene homolog
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- RAS type GTPase family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namedefender against cell death 1 pseudogene 1
-
Synonyms gene
-
Synonyms gene name
- defender against cell death 1-like
-
Synonyms
-
Locus
-
Discovery year2007-07-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
Gene info
-
Identity
-
Gene
-
Long gene nameheterogeneous nuclear ribonucleoprotein R
-
Synonyms gene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-06-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Heterogeneous nuclear ribonucleoproteins
- RNA binding motif containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameclaudin 4
-
Synonyms gene
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1998-04-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Claudins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemelanocortin 1 receptor
-
Synonyms gene name
- melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-07-27
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Melanocortin receptors
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namecatenin alpha 2
-
Synonyms gene name
- catenin (cadherin-associated protein), alpha 2
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-07-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Alpha catenins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameprotein tyrosine phosphatase receptor type K
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-02-09
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- Immunoglobulin like domain containing
-
VEGA ID
