Recombinant Mouse CD32 / FCGR2B Protein (His tag) 100µg
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Catalog number
PKSM040902-100µg
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Price
Please ask
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Size
100µg
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Activity
Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR2B-His (CD32) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml.
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Synonym
AI528646;CD32;F630109E10Rik;Fcgr2;Fcgr2a;FcgRII;Fcr-2;Fcr-3;fcRII;Fc[g]RII;Ly-17;Ly-m20;LyM-1
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Sequence
Met 1-Arg 217
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Fusion tag
C-His
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Accession
NP_001070657.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 98 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
21.7 kDa
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AP Mol Mass
35-40 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
FCGR2B, FCGR2A
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Short name
Recombinant Mouse CD32 / FCGR2B Protein (His tag) 100µg
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse CD32 / fragment c fragment on Immunoglobulin G, low affinity IIb, receptor (CD32) Protein (histidine detection labelled) 100µg
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Alternative technique
rec, murine
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Alternative to gene target
Fc fragment of IgG, low affinity IIb, receptor (CD32), CD32 and CD32B and FCG2 and FCGR2 and IGFR2, FCGR2B and IDBG-104308 and ENSG00000072694 and 2213, IgG binding, Plasma membranes
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Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIb
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIb, receptor for (CD32)
- Fc fragment of IgG, low affinity IIb, receptor (CD32)
- Fc fragment of IgG receptor IIb
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Synonyms
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GenBank acession
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Locus
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Discovery year
1991-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG receptor IIa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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