Rat Monoclonal Anti-Mouse CD117 , FITC (Clone ACK4) (rat IgG2a)
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Catalog number
MCD117-F
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Price
Please ask
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Size
100 tests
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Stock availability
Available
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Category
Primary Antibodies
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Antibody type
Monoclonal Antibody
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Antibody host
Rat
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Antibody conjugate
FITC
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Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
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Notes
The Rat Monoclonal Anti-Mouse CD117 , FITC (Clone ACK4) (rat IgG2a) is manufactured for Research Use Only or for diagnostics purposes.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This adi Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. adi FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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Gene target
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Gene symbol
KIT
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Short name
Monoclonal Anti-Mouse CD117 , FITC (Clone ACK4) ( IgG2a)
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, Monoclonals or monoclonal antibodies, mouses
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Host
Rat
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Isotype
IgG2a
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Label
FITC
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Species
Mouse, Mouses, Rats
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Alternative name
Rat monoclonal Antibody toMouse CD117 , fluorecein (clonality ACK4) (rat IgG2a)
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Alternative technique
murine, antibodies, fluorescine
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Clone name
ACK4
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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