Rabbit Polyclonal IgG Anti-Collagen Type I, Tuna fish

  • Catalog number
    CO20171-T
  • Price
    Please ask
  • Size
    0.5 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Purified collagen type I from tuna fish skin
  • Specificity
    Tunafish collagen type I
  • Species reactivity
    Tunafish, crossreactivity with collagen type I of other species
  • Product presentation
    affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
  • Product category
    Primary Antibody
  • Antibody s raised in
    Rabbit
  • Antibody s isotype
    IgG
  • Antibody s clone
    polyclonal
  • Product is supplied as
    purified, lyophilized
  • Recommended concentration for use
    IFA ≥ 1:80, IHC(P) ≥ 1:500, ELISA ≥ 1:200 (OD ≥ 500)
  • Protein concentration
    app. 1 mg/ml
  • Positive control
    Tunafish skin
  • Pretreatment
    After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  • Tested applications
    IHC(P), IFA, ELISA, RIA, IB/WB
  • Recommended secondary reagent
    Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
  • Storage method
    Store at -20°C
  • UniProt number
    nicht verfügbar
  • Litterature
    1. Rigo C., Hartmann D.J., Bairati A. - Electrophoretic and immunochemeical study of collagens from Sepia officinalis cartilage. Biochim. Biophys. Acta 2002, 1572, 77-84.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Gene
    Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    Collagen   Tuna   fish  
  • Short name
    ECM
  • Technique
    Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Species
    Fish, Fishes
  • Alternative name
    Collagen Type I, Tuna fish
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
  • Tree numbers
    • E01.370.225.500.620.670.325
    • E01.370.225.750.600.670.325
    • E05.200.500.620.670.325
    • E05.200.750.600.670.325
    • E05.393.661.475
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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