Rabbit anti Apaf 1 (NT) Apoptosis Activating Protease Fact
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Catalog numberX1118P
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Price:
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).
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Antibody come fromSynthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse
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Other descriptionProvided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti Apaf 1 (NT) Apoptosis Activating Protease Fact Antibody
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Antibody is raised inRabbit
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Antibody s reacts withHuman, Mouse, Rat
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot
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Antibody s suited forDetects Apaf-1 by Western blot at 0.25 to 0.5 µg/ml. Detects a 130 kDa band in whole HeLa cell lysate. Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Zou, H., Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997, 90, 405-13_x000B__x000B_2. Cecconi, F., et al., Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998, 94, 727-37 _x000B__x000B_3. Li, P., et al., Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997, 91, 479-89 _x000B__x000B_4. Hu, Y., et al., Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998, 95, 4386-91
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This 1 is suited for programmed cell-death studies.
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit anti Apaf 1 (NT) Apoptosis Activating Protease Fact
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Techniqueapoptosis, Rabbit, anti, antibody to, apoptosis pathway
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HostRabbit, Rabbits
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Isotypenot specified
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Labelunlabeled
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Alternative nameproduction species: rabbit antibody to Apoptotic protease activating factor 1 (NT) programmed cellular destruction Activating Protease Fact
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Alternative techniquecelldeath, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data