Mouse Anti-Rat SAPAP (pan) Monoclonal IgG2b, Clone S127-31
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Catalog numberSMC-338D
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Price:
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Size100 µg
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CloneS127-31
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ImmunogenFusion protein amino acids 772-992 (C-terminus) of rat SAPAP1
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Antibody s full descriptionMouse Anti-Rat SAPAP Monoclonal IgG2b Antibody, Clone: S127-31: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameDAP 1 ALPHA antibody, DAP 1 antibody, DAP 1 BETA antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, DAP1 antibody, Discs large (Drosophila) homolog associated protein 1 antibody, Discs large homolog associated protein 1 antibody, Disks large associated protein 1 antibody, Disks large associated protein 1 alpha isoform included antibody, Disks large associated protein 1, beta isoform, included antibody, Disks large-associated protein 1 antibody, DLGAP 1 antibody, Dlgap1 antibody, DLGP1_HUMAN antibody GKAP antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Guanylate kinase associated protein antibody, Guanylate kinase-associated protein antibody, hGKAP antibody, KIAA4162 antibody, MGC88156 antibody, mKIAA4162 antibody, PSD 95/SAP90 binding protein 1 antibody, PSD-95/SAP90-binding protein 1 antibody, SAP90/PSD 95 associated protein 1 antibody, SAP90/PSD-95-associated protein 1 antibody SAPAP 1 antibody, SAPAP1 antibody
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Raised inMouse
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Antibody s targetSAPAP
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Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~120kDa (SAPAP2), ~110kDa (SAPAP1/3/4). It does cross-react with other SAPAPs.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-338 was sufficient for detection of SAPAP 1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components (1, 2). SAPAP1, 2 and 4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies (1, 3).
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Bibliography1. Kindler S., et al. (2004) Brain Res. Mol Brain Res. 126: 14-21. 2. Bongiorno-Borbone L., et al. (2005) Biochem, Biophys. Res Commun. 337: 641-646. 3. Welch J.M., Wang D., and Fend G. (2004) J Comp. Neurol. 472: 24-39.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_075235.2
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Gene number65040
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Protein numberP97836
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Antibody s datasheetContact our support service
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Representative figure legendWestern Blot analysis of Rat brain membrane lysate showing detection of SAPAP protein using Mouse Anti-SAPAP Monoclonal Antibody, Clone S127-31 (SMC-338). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAPAP Monoclonal Antibody (SMC-338) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SAPAP Antibody [S127-31] used in Western Blot (WB) on Rat brain membrane lysate (SMC-338)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSNORD114-31, SNORD115-31, IGKV3-31, ERVK-31, IGLV3-31, RNVU1-31, IGKV3D-31, IL31, OR1D5, DEFB131A
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Short nameMouse Anti- SAPAP (pan) Monoclonal IgG2b, Clone S127-31
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelUnconjugated
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat SAPAP (pan) monoclonal IgG2b, clonality S127-31
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Alternative techniquemurine, antibodies
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Clone nameS127-31
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-31
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-31
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3-31 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3-31
- immunoglobulin kappa variable 3-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 31
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Synonyms gene name
- endogenous retrovirus group K, member 31
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GenBank acession
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Locus
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Discovery year2015-05-14
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-31 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-31
- immunoglobulin lambda variable 3-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 31
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Locus
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Discovery year2019-07-25
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-31 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-31
- immunoglobulin kappa variable 3D-31 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 31
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily D member 5
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Synonyms gene name
- olfactory receptor, family 1, subfamily D, member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Olfactory receptors, family 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedefensin beta 131A
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Synonyms gene
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Synonyms gene name
- defensin, beta 131
- defensin beta 131
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Defensins, beta
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data