Mouse Anti-Human Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80

  • Catalog number
    SMC-435D-HRP
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S330A-80
  • Immunogen
    Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
  • Antibody s full description
    Mouse Anti-Human Alpha2C Adrenergic Monoclonal IgG1 Antibody, Clone: S330A-80: HRP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Adra 2c Antibody, Alpha-2CAR Antibody, ADRA2C Antibody, ADRA2L2 Antibody, ADRARL2 Antibody, Alpha 2CAR Antibody, alpha2 AR C4 Antibody, alpha2 C4 Antibody, alpha2C Antibody, ALPHA2CAR Antibody, Alpha-2 adrenergic receptor subtype C4 Antibody, Alpha-2C adrenoreceptor Antibody, Adrenergic alpha 2C receptor Antibody, Alpha 2C adrenergic receptor Antibody, Alpha 2C adrenergic receptor subtype C4 Antibody, Alpha 2C adrenoceptor Antibody, Alpha 2C adrenoreceptor Antibody, Alpha-2-adrenergic receptor Antibody, renal type Antibody, alpha2cii-adrenergic receptor Antibody, Subtype alpha2 C4 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Alpha2C Adrenergic
  • Primary research fields
    Neuroscience, Neurotransmitter Receptors, Alpha-2 Adrenergic Receptors, Cell Signaling
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-435 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
  • Bibliography
    1. Filipeanu C.M., et al. (2011) Biochim Biophys Acta. 1813: 346-357. 2. Powe D.G., et al. (2011) Breast Cancer Res Treat. 130: 457-463.
  • Released date
    5-Feb-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_000674.2
  • Gene number
    152
  • Protein number
    P18825
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (SMC-435). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (SMC-435) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (SMC-435). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (SMC-435) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-Alpha-2C Adrenergic Receptor Antibody [S330A-80] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-435) Mouse Anti-Alpha-2C Adrenergic Receptor Antibody [S330A-80] used in Western Blot (WB) on Monkey COS cells transfected with HA-tagged Alpha-2C (SMC-435)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    The Mouse Anti- Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHV4-80, HLA-W, RNU6-80P, VNN2, UNC80, AASDHPPT, OR4H6P
  • Short name
    Mouse Anti- Alpha 2C Adrenergic Receptor Monoclonal IgG1, Clone S330A-80
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    HRP
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman a 2C Adrenergic Receptor monoclonal IgG1, clonality S330A-80
  • Alternative technique
    murine, antibodies
  • Clone name
    S330A-80
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 4-80 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable 4-80
    • immunoglobulin heavy variable 4-80 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 80, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 80
  • Locus
  • Discovery year
    2011-08-08
  • Entrez gene record
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee