Isocitrate Dehydrogenase 1 R132H mutant, Recombinant Human

• Catalog number
  P1070-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Isocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
• Alternative_names
  Isocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
• Description
  Catalyzes the rate-limiting step in Citric acid cycle
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  ≥ 300 mU/mg, in which one unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
• Results
  ≥ 300 mU/mg
• Unit Definition
  One unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
• Molecular Weight
  50.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Reconstitution Instructions
  Reconstitute in deionized water to the final concentration 0.5 mg/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
• Background Information
  Mutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Isocitrate   Dehydrogenase   R132H   mutant  
• Short name
  Isocitrate Dehydrogenase 1 R132H mutant, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Isocitrate Dehydrogenase 1 R132H mutant, Rec. H. sapiens
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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