H3K27ac, Histone H3 acetylated lysine 27 (ChIP grade)

  • Catalog number
    AS16 3195
  • Price
    Please ask
  • Size
    50 µg
  • Available ordering format
    Inquire
  • Immunogen
    KLH-conjugated synthetic peptide
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified serum in PBS with 0.05 % sodium azide
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
  • Verified applications
    chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), peptide array (PA), western blot (WB)
  • Connected products
    AS16 3190-10 | anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies AS16 3187 | anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies AS16 3182 | anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
  • Recommended dilutions for use
    0.5-5 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 500 (ELISA), 1: 500 (IF), 1: 1000 (WB)
  • Molecular weight expected аpparent
    15 kDa
  • Verified reactivity
    human
  • Possible reactivity
    A.thaliana, mouse, rat
  • No reactivity
    No confirmed exceptions from predicted reactivity known at the moment
  • Supplementary information
    Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.
  • References
    To be added when available, antibody released in February 2016.
  • Scientific context
    Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
  • Notes
    Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
  • Protein number
    P68431
  • TAIR number
    Refer to NCBI
  • Properties
    Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The agisera antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials.
  • Gene target
  • Short name
    H3K27ac, Histone H3 acetylated lysine 27 (ChIP grade)
  • Technique
    ChiP, Chromatine immuno Precipitationg antibody
  • Alternative name
    H3K27ac, Histone H3 acetylated lysine 27 (ChIP grade)
  • Alternative technique
    epigenetics
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