GM-CSF Recombinant Protein
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Catalog number
40-617
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Price
Please ask
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Size
0.02 mg
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About recombinant protein
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues. .
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Other name of the recombinant
Csfgm, GMCSF, Gm-CSf, MGI-IGM, Csfgm, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, GM-CSF
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Recombinant in
E. coli
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His tag on the recombinant protein
no His tag
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SEQUENCE
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
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Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is = 0.05 ng/mL, corresponding to a specific activity of = 2 x 107 units/mg.
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Keep at
The lyophilized GM-CSF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted GM-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing GM-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
110625658
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Accession nr
NP_034099.2
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
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About
This product is for research use only.
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Protein s origin
Murine
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Vial
Lyophilized
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NCBI ID
12981
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Gene name
colony stimulating factor 2 (granulocyte-macrophage)
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Source
Mus musculus
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Uniprot
P01587
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Gene
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Csf2
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Short name
GM-CSF Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
GM-CSF Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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