EZScreen™ ATP Colorimetric Assay Kit (384-well)
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Catalog number
K959-400
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Price
Please ask
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Size
400 assays
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Description
Highly Sensitive, minimum sample preparation required
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Summary
• Detection method- Absorbance (570 nm) • Species reactivity- All • Applications- The assay can detect as low as 80 picomol of ATP in various samples
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Detection Method
Absorbance (570 nm)
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Species Reactivity
Species reactivity-All
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Applications
BioVision’s ATP Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides ATP
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Sample Type
Cell and tissue lysates, culture media,
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Features Benefits
• Simple procedure; takes less than 1 hour • Fast and convenient • Kit is designed to be a robust method which enzymatic detection of ATP in numerous biological samples
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Adenosine Triphosphate (ATP) is a derivative of adenosine nucleotide that contains a large amount of biochemical energy stored in its high-energy phosphate bonds. ATP releases energy when it is hydrolyzed into ADP or AMP (Adenosine Di or Mono Phosphate). This energy is used by cells to drive various cellular events like metabolic processes, enzymatic reactions, motility, cell division and muscle contraction. It is also used as a substrate in signal transduction, by kinases to phosphorylate proteins and lipids, and by adenylate cyclase to produce cyclic AMP. In eukaryotic cells, ATP is produced via cellular respiration in mitochondria and by photosynthesis in chloroplasts. Anaerobic bacteria can produce ATP by breaking down nutrients into simpler metabolites, and then using the released energy to form ATP from ADP and Pi (inorganic phosphate). Aerobic bacteria also make ATP from ADP and Pi by ATPase which takes the advantage of ‘proton motive force’ when protons travel back to cytosol from periplasmic space. There are many commercially available kits that detects ATP in femtomoles or less by using luminescence but these products require specialized instrumentation and utilize luciferase which can be difficult to maintain in active form. BioVision’s EZScreenTM ATP Colorimetric Assay Kit is designed to be a robust, simple, stable, colorimetric method which utilizes a series of enzymatic reactions to form a product that is easily quantified at OD 570 nm. The method is rapid, simple, sensitive and designed for high-throughput format using a 384-well plate. The kit can detect as low as 80 pmol of ATP in a 384 well assay plate.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
VPS9D1, NFATC2
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Short name
EZScreen™ ATP Colorimetric Assay Kit (384- )
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
EZScreen™ adenosine triphosphate colorimetry test reagent (384-well)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
VPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Product images
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