CD45 (Clone:T29/33), RPE-Cy5 + Mouse IgG1 (Clone:DAK-G01), FITC + Ms IgG1 (Clone:DAK-G01), RPE, Neg. Cont., tri-label
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Catalog numberAXL1087
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PricePlease ask
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Size500ul
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Alternative name 1CD45 (Clone:T29/33), RPE-Cy5 + Mouse IgG1 (Clone:DAK-G01), FITC + Ms IgG1 (Clone:DAK-G01), RPE, Neg. Cont., tri-label
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Alternative name 2CD45 (Clone:T29/33), RPE-Cy5 + Mouse IgG1 (Clone:DAK-G01), FITC + Ms IgG1 (Clone:DAK-G01), RPE, Neg. Cont., tri-label
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Alternative name 3CD45 (Clone:T29/33), RPE-Cy5 + Mus musculus IgG1 (Clone:DAK-G01), FITC + Ms IgG1 (Clone:DAK-G01), RPE, Neg. Cont., tri-label
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Alternative name 4anti-CD45 Clone:T29/33 + IgG1 Clone:DAK-G01 + Ms IgG1 Clone:DAK-G01 Neg. Cont. tri-label
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Alternative name 5CD45 Clone:T29/33 + IgG1 Clone:DAK-G01 + Ms IgG1 Clone:DAK-G01 Neg. Cont. tri-label
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesCy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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ConjugationCy5.5, Anti-FITC Antibody
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Additional descriptionBrady printing labels are printed by a label printer for labeling with 6,5 m long cassettes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolRPE, TKFC, PTPRCAP, PTPRC
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Short nameCD45 (Clone:T29/33), RPE- + Mouse IgG1 (Clone:DAK-G01), FITC + Ms IgG1 (Clone:DAK-G01), RPE, Neg. Cont., tri-
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TechniqueMouse, FITC, Fluorescein, mouses
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Hostmouse
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IsotypeIgG1
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Labelrpe
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SpeciesMouse, Mouses
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Alternative nameCD45 (clonality:T29/33), RPE-cyanine 5 + Mouse IgG1 (clonality:DAK-G01), fluorecein + Ms IgG1 (clonality:DAK-G01), RPE, Neg. Cont., tri-label
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Alternative techniquemurine, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametriokinase and FMN cyclase
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Synonyms gene
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Synonyms gene name
- dihydroxyacetone kinase 2 homolog (yeast)
- dihydroxyacetone kinase 2 homolog (S. cerevisiae)
- triokinase/FMN cyclase
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Synonyms
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Synonyms name
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Locus
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Discovery year2005-10-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data