Annexin V Apoptosis Detection Kit with PI-Biotin

  • Catalog number
    ANXVKB-100T
  • Price
    Please ask
  • Size
    100 TEST
  • Description
    Annexin V Apoptosis Detection Kit with PI-Biotin is a kit used for the identifacation and detection of apoptotic and necrotic cells, this Kit was conjugated with Biotin format and reported for use in flow cytometric analysis, it reacts with Annexin V proteins, with Propidium Iodide Solution (PI) helps to make the difference between apoptic cells and necrotic, used also as a dead cell exclusion dye.
  • Other name
    Annexin A5 Apoptosis Detection Kit
  • Application
    Flow Cytometry Method
  • Conjugate
    Biotin
  • Reactivity
    All mammalian
  • Formulation
    Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Expiration
    After 1 year
  • Storage
    2-8°C, store in dark, do not freeze
  • Remark
    For Recherche Use Only.
  • Additional description
    This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
    Annexin   Kit   with  
  • Short name
    Annexin V Apoptosis Kit with PI-
  • Technique
    apoptosis, detection, apoptosis pathway
  • Label
    Biotin
  • Alternative name
    Annexin V programmed cellular destruction quantification reagent including PI-biotinilated
  • Alternative technique
    celldeath, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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