Active SIRT6 (His-tagged), human recombinant

• Catalog number
  7699-100
• Price
  Please ask
• Size
  100 µg

• Synonyms
  SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
• Alternative_names
  SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
• Description
  His tagged Human SIRT6 from E.Coli, AOF, ≥90% pure, biologically active.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  BioVision’s Active His-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C).
• Biological activity
  >3 mU/mg
• Results
  >3 mU/mg
• Unit Definition
  One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C
• Molecular Weight
  Human SIRT6 is a 41.3 kDa (1-355 aa + N-terminal polyhistidine tag) protein. It runs as ~30 kDa during SEC and ~47 kDa on a SDS-PAGE.
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  Human SIRT6 protein is a solution of 1 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20% glycerol.
• Background Information
  Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-α.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   SIRT6   His-tagged  
• Gene symbol
  SIRT6
• Short name
  Active SIRT6 (His-tagged), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  his-tagged
• Species
  Human, Humans
• Alternative name
  functionnal SIRT6 (histidine-tagged), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:14934
• Gene
  SIRT6
• Long gene name
  sirtuin 6
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• GenBank acession
  • AF233396
• Locus
  19p13.3
• Discovery year
  2001-03-20
• Entrez gene record
  51548
• Pubmed identfication
  • 10873683
• Classification
  • Sirtuins
• VEGA ID
  OTTHUMG00000181847

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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