Rabbit anti Acinus (NP)
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Catalog numberX1105P
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Price:
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionChromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusSÕ (2).
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Antibody come fromSynthetic peptide (DDPVRTAQVPSPPRGK) corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusSÕ, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the N-terminus of active peptide p17.
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Other descriptionProvided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti Acinus (NP) Antibody
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Antibody is raised inRabbit
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot
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Antibody s suited forDetects Acinus by Western blot at 0.5 to 1.0 µg/ml. Detects a 220 kDa band in K562 cell lysate. Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Condensed matter in cell death. Zamzami, N and Kroemer, G. , Nature, 1999 , 401, 127-8_x000B__x000B_2. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Sahara S, et al., Nature, 1999, 401, 168-73
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit anti Acinus (NP)
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TechniqueRabbit, anti, antibody to
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HostRabbit, Rabbits
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Isotypenot specified
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Labelunlabeled
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Alternative nameproduction species: rabbit antibody to Acinus (NP)
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Alternative techniquerabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data