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Category
Primary Antibodies
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Long description
TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases,gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation,branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.
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Antibody come from
Synthetic peptdide derived from the human ELOV4 protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
3A4
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Antigen antibody binding interaction
Mouse anti Tissue Inhibitor of Matrix Metalloproteinase 2 (TIMP2) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rabbit, Chicken, Zebrafish, Pig
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA, Western Blot, Immunohistochemistry
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Antibody s suited for
Antibody can be used for Western blotting (1-5ʵg/ml starting dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1: Kobayashi T, et al. ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3-L1 and F442A cells. FEBS Lett. 2007 Jul 10;581(17):3157-63. Epub 2007 Jun 11. _x000B_2: DeAngelis MM, et al. Cigarette smoking, CFH, APOE, ELOVL4, and risk of neovascular age-related macular degeneration. Arch Ophthalmol. 2007 Jan;125(1):49-54. _x000B_3: Vasireddy V, et al. Loss of functional ELOVL4 depletes very long-chain fatty acids (> or =C28) and the unique omega-O-acylceramides in skin leading to neonatal death._x000B_Hum Mol Genet. 2007 Mar 1;16(5):471-82. Epub 2007 Jan 5. _x000B_4: Sridharan S, et al. X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB). J Mol Biol. 2007 Feb 16;366(2):469-80. Epub 2006 Nov 7. _x000B_5: Seitsonen S, et al. Analysis of variants in the complement factor H, the elongation of very long chain fatty acids-like 4 and the hemicentin 1 genes of age-related macular degeneration in the Finnish population. Mol Vis. 2006 Jul 20;12:796-801. _x000B_6: Jakobsson A, et al. Fatty acid elongases in mammals: their regulation and roles in metabolism. Prog Lipid Res. 2006 May;45(3):237-49. Epub 2006 Mar 6. Review. _x000B_7: Pleite R, et al. Inhibitors of fatty acid biosynthesis in sunflower seeds. J Plant Physiol. 2006 Sep;163(9):885-94. Epub 2006 Feb 28. _x000B_8: Kajikawa M, et al. Isolation and functional characterization of fatty acid delta5-elongase gene from the liverwort Marchantia polymorpha L. FEBS Lett. 2006 Jan 9;580(1):149-54. Epub 2005 Dec 6. _x000B_9: Koo AJ, et al. Identification of a plastid acyl-acyl carrier protein synthetase in Arabidopsis and its role in the activation and elongation of exogenous fatty acids. Plant J. 2005 Nov;44(4):620-32. _x000B_10: Zhang K., et al. A 5-bp deletion in ELOVL4 is associated with two related forms of autosomal dominant macular dystrophy.; Nat. Genet. 27:89-93(2001)._x000B_11: Edwards A.O., et al; A novel gene for autosomal dominant Stargardt-like macular dystrophy with homology to the SUR4 protein family.; Invest. Ophthalmol. Vis. Sci. 42:2652-2663(2001).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme. 6
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
TIMP2
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Short name
Mouse anti Tissue Inhibitor of Matrix Metalloproteinase 2 (TIMP2)
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Technique
tissue, Mouse, anti, antibody to, mouses, tissues
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Host
mouse
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Isotype
IgG2a
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Tissue suppressor on Matrix Metalloproteinase 2 (TIMP metallopeptidase inhibitor 2)
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Alternative technique
tissues, murine, antibodies
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Alternative to gene target
TIMP metallopeptidase inhibitor 2, CSC-21K and DDC8, TIMP2 and IDBG-70963 and ENSG00000035862 and 7077, metal ion binding, Extracellular, Timp2 and IDBG-214632 and ENSMUSG00000017466 and 21858, BT.52974 and IDBG-642791 and ENSBTAG00000010899 and 282093
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Tissue
tissue
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