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Mouse anti LEF-1 all isoforms

  • Catalog number
    X1076M
  • Product name
    Mouse anti LEF-1 all isoforms
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.
  • Antibody come from
    Recombinant full length LEF-1 protein
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    1C3.1D10
  • Isotype
    IgG1
  • Antigen antibody binding interaction
    Mouse anti LEF-1 all isoforms Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot
  • Antibody s suited for
    Antibody detects all LEF-1 isoforms by Western blot. Does not cross-react with TCF-1 or TCF-4. Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Riese, J., et al., LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic. Cell 1997, 88, 777-787_x000B__x000B_2. Giese, K., et al. Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1. Proc. Natl. Acad. Sci. USA 1997, 94, 12845-12850_x000B__x000B_3. Behrens, J., et al. Functional interaction of beta-catenin with the transcription factor LEF-1. Nature 1996, 382, 638-642_x000B__x000B_4. Giese, K. and Grosschedl, R. LEF-1 contains an activation domain that stimulates transcription only in a specific context of factor-binding sites. EMBO J. 1993, 12, 4667-4676
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    LEF-1 all isoforms
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    LEF1-AS1, LEF1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse anti LEF-1 all isoforms
  • label filter
    • unlabeled
  • technique filter
    • Mouse
    • anti
  • Technique
    Mouse, anti, antibody to, mouses
  • Alternative technique
    murine, antibodies