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Category
Primary Antibodies
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Long description
Anti-Human Immunodeficiency Virus Reverse Transcriptase is an antibody meant to target the Reverse Transcriptase (RT) efficiency in HIV infections. RT allows the viral RNA to be transcribed to host DNA and synthesize new DNA composed of the virusÕ genome. Because RT also exhibits RNaseH activity, it enables the production of a DNA/RNA hybrid used for synthesis. Additionally, it can cause errors during transcription, creating difficulties in isolating the virus and promoting further infection.
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Antibody come from
Recombinant immuno-deficiency virus reverse transcriptase (HIV- RT).
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Other description
Provided as a 0.2 µm sterile-filtered solution in Ca++ & Mg++ free Dulbecco's PBS with 0.08% sodium azide.
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Clone
AS34
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Antigen antibody binding interaction
Mouse anti HIV reverse transcriptase (HIV-RT) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
n/a
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunoprecipitation, Enzyme Immunoassay
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Antibody s suited for
Applications: ELISA: This antibody has been used as a coating antibody at 10 µg/ml in a two site ELISA with AS35. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of 90% HIV-RT in solution using a double-antibody method (Midgley and Hepburn). Immunoprecipitation has also been accomplished using Protein G agarose.
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Storage
-20ºC
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Relevant references
Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Gene
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Goup
reverse transcription
