Mouse anti CD7 antibody conjugated to FITC

• Catalog number
  0072
• Price
  Please ask
• Size
  100 Tests

• Category
  Primary Antibodies
• Long description
  Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cells. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.
• Antibody come from
  CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells.
• Other description
  Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
• Clone
  MG34
• Antigen antibody binding interaction
  Mouse anti CD7 antibody conjugated to FITC Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  CD Marker
• Application
  Flow Cytometry
• Antibody s suited for
  PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  4ºC
• Relevant references
  1. Isolation and characterization of the genomic human CD7 gene: structural similarity with the murine Thy-1 gene. Schanberg, L.E., Fleener, D.E., Kurtzberg, J., Haynes, B.F., Kaufman, R.E.; Proc. Natl. Acad. Sci. USA , 1991 Jan 1;88(2):603-7. _x000B__x000B_2. Identification of novel B-lineage cells in human fetal bone marrow that coexpress CD7. Grumayer, E.R., Griesinger, F., Hummell, D.S., Brunning, R.D., Kersey, J.H.; Blood, 1991 Jan ; 77(1):64-8. _x000B__x000B_3. Genuine CD7 expression in acute leukemic and lymphoblastic lymphoma. Osada, H., Emi, N., Ueda, R., Seto, M., Koike, K., Suchi, T., Kojima, S., Obata, Y., Takahashi, T.; Leuk. Res. 1990;14(10):869-77. _x000B__x000B_4. Inhibition of alloresponsive naive and memory T cells by CD7 and CD25 antibodies and by cyclosporine. Akbar, A.N., Amlot, P.L., Ivory,K., Timms, A., Janossy, G.; Transplantation, 1990 No;50(5):823-9. _x000B__x000B_5. Comparsion of outcome, clinical, laboratory, and immunological features in 164 children and adults with T-ALL. Garand, R., Vannier, J.P., Bene, M.C., Faure, G., Favre, M., Bernard, A,. Leukemia,1990 No;4(11):739-44.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  CD7   conjugated  
• Gene symbol
  CD7
• Short name
  Mouse anti CD7 antibody conjugated FITC
• Technique
  Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG2a
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD7 molecule (antibody to-) coupled to fluorecein
• Alternative technique
  antibodies, murine, fluorescine
• Alternative to gene target
  CD7 molecule, GP40 and LEU-9 and Tp40 and TP41, CD7 and IDBG-73437 and ENSG00000173762 and 924, protein binding, Plasma membranes, Cd7 and IDBG-214932 and ENSMUSG00000025163 and 12516, CD7 and IDBG-642137 and ENSBTAG00000011359 and 510073

Gene info

• Identity
  HGNC:1695
• Gene
  CD7
• Long gene name
  CD7 molecule
• Synonyms gene name
  • CD7 antigen (p41)
• Synonyms
  • GP40
  • LEU-9
  • TP41
  • Tp40
• Synonyms name
  • p41 protein
  • T-cell antigen CD7
  • T-cell leukemia antigen
• GenBank acession
  • X06180
• Locus
  17q25.3
• Discovery year
  1986-01-01
• Entrez gene record
  924
• Pubmed identfication
  • 1695199
  • 3501369
• RefSeq identity
  • NM_006137
• Classification
  • CD molecules
  • V-set domain containing
• VEGA ID
  OTTHUMG00000178662

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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