Mouse anti alpha SNAP
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Catalog numberX1026
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Price:
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionSNAPs (soluble NSF attachment proteins),acting in concert with SNAREs _x000B_(SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicleto-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. -SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas -SNAP is expressed in all mammalian tissues, -SNAP is expressed only in brain.
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Antibody come fromBalb/C mice were immunized with recombinant human -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The antibody was purified to >95% homogeneity by standard chromatographic techniques
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Other description0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been included in the antibody solution.
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Clone15D4
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Antigen antibody binding interactionMouse anti alpha SNAP Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman, Rat, Bovine
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationWestern Blot, Immunoprecipitation, Enzyme Immunoassay,
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Antibody s suited forOptimal concentration should be evaluated by serial dilutions. Clone 4E4 anti- SNAP specifically recognizes -SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize -SNAP_x000B__x000B_Application: A dilution of 0.5-5 ug/ml is suggested for western blot and 2-10 ug/ml for immunoprecipitation.
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Storage-20ºC
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThe Mouse anti alpha SNAP is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolNAPB, SNAP25, SNAP29, SNAP47
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Short nameMouse anti alpha SNAP
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to a SNAP
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameNSF attachment protein beta
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Synonyms gene name
- N-ethylmaleimide-sensitive factor attachment protein, beta
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptosome associated protein 25
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Synonyms gene
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Synonyms gene name
- synaptosomal-associated protein, 25kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year1995-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SNAREs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptosome associated protein 29
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Synonyms gene name
- synaptosomal-associated protein, 29kDa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SNAREs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptosome associated protein 47
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Synonyms gene
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Synonyms gene name
- chromosome 1 open reading frame 142
- synaptosomal-associated protein, 47kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-07-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data