Goat Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM IgD (heavy and light chains)

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• Catalog number
  GAM/Ig(GAMD)
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• Size
  1 mL

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• Category
  Secondary Antibodies
• Long description
  The reactivity of the antiserum is directed to the heavy chains of immunoglobulin isotypes IgG, IgA, IgM and IgD. The antiserum also contains antibodies to common determinants and to the surface determinants of the common Fab as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig proteins in mouse serum. In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of immunoglobulins in mouse serum or other body fluids; to prepare an immunoadsorbent for the purification of mouse immunoglobulins from serum or plasma; as secondary antibody to precipitate the immunoglobulin in normal mouse serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
• Antibody come from
  Purified polyclonal mouse IgG and pools of homogenous IgG, IgA, IgM and IgD. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Other description
  Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Goat Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM IgD (heavy and light chains) Antibody
• Antibody is raised in
  Goat
• Antibody s reacts with
  Mouse
• Antibody s reacts with these species
  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Antibody s specificity
  Precipitating polyclonal Goat antiSerum to Mouse immunoglobulins IgG, IgA, IgM and IgD, heavy and light chains
• Research interest
  Veterinary
• Application
  Immunoprecipitation
• Antibody s suited for
  Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:32 when tested against normal mouse serum in agar immunodiffusion block titration.
• Storage
  The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Latin name
  Capra aegagrus hircus, Mus musculus
• Properties
  Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of mature B-lymphocytes where it is usually coexpressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum for virus detection. Anti-viral immunoglobulin D antibodies have a diagnostic value. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

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• Gene target
  IgG1   IgG2a   IgG2b   IgG3   heavy   light   chains  
• Short name
  Goat Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM IgD (heavy light chains)
• Technique
  light, Mouse, Goat, IgA, IgD, IgM, goats, IgAs, IgDs, IgMs, mouses
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  not specified, IgA, IgD, IgG1, IgG2a, IgG2b, IgG3, IgM
• Label
  unlabeled
• Species
  Mouse, Goats, Mouses
• Alternative name
  caprine Mouse IgG1 IgG2a IgG2b IgG3 Immunoglobulin A Immunoglobulin M IgD (heavy and light chains)
• Alternative technique
  murine, caprine, immunoglobulin-a, immunoglobulin-d, immunoglobulin-m

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