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Goat anti Mouse IgG2a (subclass specific)

  • Catalog number
    GAM/IgG2a/7S
  • Product name
    Goat anti Mouse IgG2a (subclass specific)
  • Size
    10 mg
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the subclass IgG2a as tested against mouse sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5.000.
  • Antibody come from
    Pools of purified homogenous IgG2a isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgG2a
  • Antigen antibody binding interaction
    Goat anti Mouse IgG2a (subclass specific) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this product has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate. If the IgG fraction is to be used in the presence of material originating from another species, prior screening for cross-reactivity is essential an additional adsorption of the reagent may required to ensure intra-assay specificity. In double radial immunodiffusion a reaction with Rat has been observed.
  • Label
    unlabeled
  • Antibody s specificity
    IgG fraction of polyclonal Goat antiSerum to Mouse IgG2a, subclass specific
  • Research interest
    Veterinary
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody s suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
  • Storage
    The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    IgG2a subclass specific
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti Mouse IgG2a (subclass specific)
  • label filter
    • unlabeled
  • technique filter
    • anti mouse
    • Mouse
    • anti
    • Goat
  • Technique
    anti mouse, Mouse, anti, Goat, antibody to, anti mouse antibodies, goats, mouses
  • Alternative technique
    murine, antibodies, caprine