Goat anti Monkey C3c

#

• Catalog number
  GAMon/C3c/7S
• Price:

  Please ask

  Ask for price
• Size
  10 mg

# #

• Category
  Primary Antibodies
• Long description
  In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other protein components of monkey serum or plasma. As unlabelled primary or secondary antibody reagent for the indirect detection of C3c in monkey cells, tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:25 and 1:250; in ELISA and comparable nonprecipitating antibody-binding assays between 1:50 and 1:500.
• Antibody come from
  C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Other description
  Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Goat anti Monkey C3c Antibody
• Antibody is raised in
  Goat
• Antibody s reacts with
  Monkey
• Antibody s reacts with these species
  The antiSerum does not cross-react with any other component of Monkey plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. In addition to a clear reactivity with C3c of other old-world Monkeys (Cercopithecus, Cynomolgus and Baboon), this antiSerum shows also a fair amount of cross-reactivity to C3C of other species, including Chimpanzee and man.
• Antibody s specificity
  Purified IgG fraction of polyclonal Goat antiSerum to C3c fragment of Monkey complement factor C3
• Research interest
  Veterinary
• Application
  Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
• Antibody s suited for
  Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Precipitin titre 1:8 when tested against pooled normal monkey serum in agar-block immunodiffusion titration.
• Storage
  The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.
• Latin name
  Capra aegagrus hircus

#

• Gene target
  C3c  
• Short name
  Goat anti C3c
• Technique
  anti, Goat, antibody to, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  not specified
• Label
  unlabeled
• Species
  Monkey, Goats, Monkeys
• Alternative name
  caprine antibody to Monkey C3c
• Alternative technique
  antibodies, caprine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products