Recombinant Staphylococcus aureus D-alanine--D-alanine ligase (ddl)

Products_type

Recombinant Protein
Products_short_name

[D-alanine--D-alanine ligase (ddl)]
Other_names

[MULTISPECIES: D-alanine--D-alanine ligase; D-alanine--D-alanine ligase; D-Ala-D-Ala ligase]
Products_gene_name

[ddl]
Other_gene_names

[ddl]
Purity

>85% (SDS-PAGE)
Form

Lyophilized or liquid (Format to be determined during the manufacturing process)
Storage_stability

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Staphylococcus aureus D-alanine D-alanine ligase ddl
Short name

Recombinant Staphylococcus aureus D-alanine--D-alanine ligase (ddl)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

E Coli or Yeast or Baculovirus or Mammalian Cell
Alternative name

Rec. Staphylococcus aureus D-alanine--D-alanine ligase (ddl)
Alternative technique

rec

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data