RAT ANTI MOUSE IgG2b HEAVY CHAIN:FITC
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Catalog numberMBS235022
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Price:
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Size5x0.5 mg
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Products_typeAntibody
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Products_short_name[IgG2b]
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Other_names[Ig gamma-2B chain C region, partial; Ig gamma-2B chain C region]
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Products_gene_name[IgG2b]
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Other_gene_names[Igh-3]
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ClonalityMonoclonal
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Clone[LO-MG2b-2]
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FormFITC Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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ConcentrationIgG concentration 1 mg/ml
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Storage_stabilityStore at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_applicationFlow cytometry (FC/FACS)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolFCGRT
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Short nameANTI MOUSE IgG2b HEAVY CHAIN:FITC
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Techniqueanti mouse, Mouse, anti, FITC, antibody to, anti mouse antibodies, Fluorescein, mouses
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HostRat
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IsotypeIgG1, IgG2b
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameRAT antibody to MOUSE IgG2b HEAVY epitope:fluorecein
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Alternative techniquemurine, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameFc fragment of IgG receptor and transporter
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Synonyms gene name
- Fc fragment of IgG, receptor, transporter, alpha
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-08-23
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Entrez gene record
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Pubmed identfication
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Classification
- C1-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data