Mouse Stem Cell Factor Recombinant
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Catalog numberMBS553178
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Price:
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Size0.01 mg
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Products_typeRecombinant Protein
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Products_short_name[Stem Cell Factor]
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Products_name_syn[Mouse Stem cell Factor; kit ligand; kitlg; mgf; stem cell factor; scf]
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Other_names[kit ligand; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand]
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Products_gene_name[SCF]
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Other_gene_names[Kitlg; Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG]
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Purity>95%, as determined by SDS-PAGE and HPLC
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FormRecombinant Stem cell factor was lyophilized from a 0.2 um filtered solution in 20mM Tris, 5% trehalose, pH 7.5.
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Storage_stabilityThe lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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DescriptionFor cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Latin nameMus musculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolKITLG, TFDP1, DIPK2A
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Short nameMouse Stem Factor Recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostInsect Cells
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SpeciesMouse, Mouses
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Alternative nameMouse progenitor cellular Factor Rec.
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Alternative techniquerec, murine
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Tissuecell, stem
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Gene info
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Identity
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Gene
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Long gene nameKIT ligand
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nametranscription factor Dp-1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-02-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transcription factor Dp family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedivergent protein kinase domain 2A
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Synonyms gene
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Synonyms gene name
- chromosome 3 open reading frame 58
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-02-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- divergent protein kinase domain family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data