Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-SPRD

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• Catalog number
  MBS674722
• Price:

  501.13 USD

  Ask for price
• Size
  0.25 mg

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• Products_type
  Secondary Antibody
• Products_short_name
  [Goat F(ab')2 Anti-Rat IgG (H+L chain specific)]
• Products_name_syn
  [Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads - SpectralRed-Labeled; Goat F(ab')2 Anti-Rat IgG (H+L chain specific) (Absorbed against mouse immunoglobulins)]
• Clonality
  Polyclonal
• Reactivity
  Rat
• Specificity
  Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with mouse immunoglobulins.
• Purity
  Gel filtration of pepsin-digested antibody.
• Form
  SpectralRed (SPRD) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), and R-phycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  f(ab')2s
• Latin name
  Capra aegagrus hircus, Mus musculus, Rattus norvegicus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

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• Gene target
  H+L   ads-SPRD  
• Short name
  Goat F(ab')2 Anti- IgG (H+L), Mouse ads-SPRD
• Technique
  F(ab')2, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies,  F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. goats, IgGs, mouses
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
• Label
  SPRD
• Species
  Mouse, Goats, Mouses, Rats
• Alternative name
  caprine F(Antibody')2 Antibody toRat Immunoglobulin G (heavy + light chain), Mouse ads-SPRD
• Alternative technique
  secondairy, murine, antibodies, caprine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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