Blocking Buffer for Lateral Flow[Blocking Buffer for Lateral Flow Assays, PBS based]
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Catalog number
MBS318406
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Price
5.54 USD
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Size
NA
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Products_type
Reagent
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Test
You can block the antibody by the specific target amino acid sequence of peptide. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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Description
Buffering solutions are useful to keep the pH range sable when using this reagent of MyBioSource.
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Buffer
Phosphate-buffered saline (abbreviated PBS) is a buffer solution and Phosphate Buffered Saline (PBS buffer), pH 7.4, stabilized is a water-based salt solution containing sodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate.
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Gene target
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Short name
Blocking for Lateral [Blocking for Lateral Assays, PBS based]
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Technique
Blocking, lateral, Flow, PBS, blocking peptides, Phosphate Buffered Saline
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Alternative name
inhibiting solution to measure Lateral Flow[inhibiting solution to measure Lateral Flow tests, Phosphate buffered saline based]
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Alternative technique
control, phosphatebuffer
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MeSH Data
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Name
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Concept
Scope note:
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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