MTC Bio

MTC Bio is a laboratory equipment and consumables supplier based in Sayreville, New Jersey, USA. Since its establishment in 2009, the company has been dedicated to providing life science laboratories with innovative products designed to enhance efficiency, safety, and results.

Product Portfolio:

Liquid Handling:
HALO™ Pipettors: Lightweight pipettors with effortless plunger strokes, available in various sizes, including an exclusive 1250µL model.
ProPette™ Pipette Controllers: Electronic and manual pipette controllers designed for precision and ease of use.
Sample Storage:
ClearSeal™ Screw Cap Microtubes: Microtubes with silicone O-rings and clear graduations, available in seven sizes for secure sample storage.
Freezer/Storage Boxes: Suitable for temperatures as low as -80˚C, featuring translucent lids and numbered cavities for organized storage.
Lab Safety and Organization:
BenchBin™ Biohazard Bin: Stylish and functional disposal bins for contaminated materials like tips, tubes, and gloves.
BowTie™ Biohazard Bags: Autoclavable bags with drawstrings for safe disposal of biohazardous waste.
Cell Straining:
SureStrain™ Premium Cell Strainers: Designed for efficient filtration of cell suspensions, compatible with standard 50mL tubes.
PCR Supplies:
DoubleDecker™ PCR Freezer Boxes: Innovative storage solutions for PCR tubes and plates, optimizing freezer space.

Innovative Solutions:

nUVaClean™ UV Pipette Sterilizer: Utilizes UV light to sterilize pipettors, reducing the risk of cross-contamination.
LinkLabel™ Bluetooth Enabled Labeler: Facilitates efficient labeling of laboratory samples with wireless connectivity.

Browse MTC Bio Products

10mL Centrifuge Tubes

SKU: C2410|Size: 1000 Units/Case

250mL Tube Rack

SKU: R1900|Size: 5 Units/Pack

5mL CapLock

SKU: C2586|Size: 20 Units/Pack

5mL Centrifuge Adapters

SKU: C2570|Size: 4 Units/Pack

5mL MacroTubes®

SKU: C2500-AST|Size: 200 Sheets/Pack

5mL MacroTube® Rack

SKU: C2590|Size: 1 Each

5mL MacroTube® Vivid™

SKU: C2545|Size: 500 Units/Case

Adapter for SureStrain™ Premium Cell Strainers

SKU: C4015|Size: 25 Units/Pack

Aluminum Sealing Film

SKU: P1001-A|Size: 100 Units/Pack

Amber Screw-cap Microtube

SKU: C2230-AM|Size: 1000 Units/Case

Assay Plates

SKU: DP110F-384-BLK|Size: 100 Units/Case

Autoclave & Incubator Rack

SKU: R3015|Size: Inquire

Autoclave Bags

SKU: A9000C|Size: 200 Units/Case

Automation Reservoirs

SKU: RES09MC-12-N|Size: 50 Pack/Case

Autoradiography Film

SKU: A8803|Size: 100 Sheets/Box

BenchBin 3L™ Starter Kit

SKU: A8403-SK|Size: 1 Each

BenchBin™ Benchtop Biohazard Bin

SKU: A8401B|Size: 1 Each

BenchBin™ Starter Kit

SKU: A8401-SK|Size: 1 Each

BenchBin™3L Benchtop Biohazard Bin

SKU: A8403B|Size: 1 Each

Benchtop Disposal Set

SKU: A9004-SK|Size: 1 Each

BetterBox™ Microtube Storage Boxes

SKU: R1081-A|Size: 5 Units/Pack

Bio Bucket™

SKU: B8300|Size: 1 Each

BioWand™ Personal UV Sanitizer

SKU: U1001-W|Size: 1 Each

BowTie™ Biohazard Bags

SKU: A8001R|Size: 100 Sheets/Pack

BowTie™ Biohazard Bin

SKU: A8000B|Size: 1 Each

Cap inserts for cryogenic vials

SKU: V5809-W|Size: 500 Units/Pack

Caps for Culture Tubes

SKU: T8309|Size: 1000 Units/Case

Caps for FlowTubes™

SKU: T9009|Size: 500 Units/Pack

Carboard Freezer Boxes

SKU: R2781-P|Size: 5 Units/Pack

Cardboard Freezer Box

SKU: R3700|Size: 50 Units/Case

Cardboard Freezer Boxes

SKU: R2781-R|Size: 5 Units/Pack

Carousel Stand for Halo™ Pipettes

SKU: H6800-CR|Size: 1 Each

Centrfiuge Tubes

SKU: C2607|Size: 500 Units/Case

Centrifuge Tubes

SKU: C2630|Size: 500 Units/Case

ClearZone™ Sealing Film

SKU: SF063PP-96-CZ|Size: 500 Units/Case

CoolCaddy™ PCR WorkStation

SKU: R4015|Size: 1 Each

Cryo Storage Box

SKU: R2081|Size: Inquire

Culture Tubes

SKU: T8306|Size: 1000 Units/Case

Disposable Cuvette

SKU: S4100-1|Size: 1000 Units/Case

Disposable Cuvette Caps

SKU: S4100-5|Size: 500 Units/Pack

Double Decker™ PCR Box

SKU: R2392|Size: 5 Units/Pack

DuoClick™ Screw-Cap Culture Tubes

SKU: T8730|Size: 500 Units/Case

ELISA Sealing Film

SKU: P1001-E|Size: 100 Units/Pack

Erlenmeyer Flasks

SKU: F4061-F|Size: 12 Units/Case

Externally Threaded Cryogenic Vials

SKU: V3803|Size: 500 Units/Case

Flip Top Vial / Digestion Tube

SKU: T0050|Size: 500 Units/Case

FlowTubes™ Flow Cytometry/FACS Tubes

SKU: T9010|Size: 500 Units/Case

Freezer Box

SKU: C2580-25|Size: 5 Units/Pack

Gel Crusher™ Spin Column

SKU: C6150|Size: 200 Units/Pack

Glass-Coated Multi-Well Plates

SKU: DP04UR-9-GC10|Size: 10 Plates/Pack

Halo™ Pipettor Starter Kit

SKU: H6800-SK|Size: 1 Each

Halo™ Single Channel Pipettor

SKU: H6800-100|Size: 1 Each

HotGuard™ Autoclave Gloves

SKU: A8095|Size: 1 Each

HyperStretch™ Sealing Film

SKU: M0381|Size: 1 Each

IceRacks

SKU: R8013|Size: 6 Units/Case

Individually Wrapped Screw-cap Microtube

SKU: C2231-W|Size: 500 Units/Case

Inoculating Loops

SKU: M4025|Size: 1000 Units/Case

Inoculating Needles

SKU: M4030|Size: 1000 Units/Case

Internally Threaded Cryogenic Vials

SKU: V4801|Size: 500 Units/Case

LABeler™ Hand Held Labeler

SKU: L9010|Size: 1 Each

Large Biohazard Bin

SKU: A8002B|Size: 1 Each

LinkLabel™ Bluetooth Enabled Labeler

SKU: L3000|Size: 1 Each

LoadWell™ Gel Loading Pipet Tip

SKU: P5720|Size: 960 Units/Pack

Locking rack for cryogenic vials

SKU: V5920|Size: 4 Units/Case

Micro Centrifuge Tubes

SKU: C2007|Size: 500 Sheets/Pack

Microbiological Spreaders

SKU: M5441|Size: 100 Units/Pack

Microinjection Pipette Tips

SKU: P5710|Size: 96 Units/Pack

Microscope Slide Dispenser

SKU: M7100-SD|Size: 1 Each

Microscope Slides

SKU: M7100-90|Size: 72 Units/Pack

Multi-Well Plate

SKU: DP24VS-384-N|Size: 50 Pack/Case

Multi-Well Plates

SKU: DP04UR-9-N|Size: 100 Units/Case

Multichannel Solution Reservoirs

SKU: P8208|Size: 100 Units/Case

MyFuge™ 5D Mini-Centrifuge

SKU: C2595|Size: 1 Each

NUVaClean™ UV Pipette Carousel

SKU: P5590-E|Size: 1 Each

OptiWell™ Automation Reservoirs

SKU: D3390-8|Size: 50 Units/Case

OptiWell™ Deep Well Plates

SKU: D3096-35S|Size: 50 Units/Case

OptiWell™ DeepWell Plates

SKU: D3384-01S|Size: 100 Units/Case

OptiWell™ Sealing Mats for DW Plates

SKU: D3320-5S|Size: 50 Units/Pack

OptiWell™ Tip Combs

SKU: D3340-08|Size: 480 Units/Case

PCR 8-Strips

SKU: PC0208-TF-ATT|Size: 600 Units/Case

PCR Plates

SKU: P9602-S|Size: 50 Units/Pack

PCR Sealing film

SKU: P1001-PCR|Size: 100 Units/Pack

PCR Strip Tubes

SKU: P3020|Size: 120 Units/Pack

PCR Tools

SKU: PC-COOL-TOOL-1|Size: 1 Each

PCR Tube Opening Tool

SKU: P0800-TL|Size: 2 Units/Pack

PCR Tubes

SKU: P3030|Size: 1000 Units/Case

PTFE-Coated Sealing Mats

SKU: MAT-PC-96-PTFE|Size: 50 Pack/Case

Pestle for FlowTubes™

SKU: T9045|Size: 100 Units/Case

Pipette Carousel Rack

SKU: P4280-CR|Size: 1 Each

Pipette Rack

SKU: P4408|Size: 1 Each

Pipette Tips

SKU: P4300-RK|Size: 960 Units/Pack

Pipette Tips for Halo™ Pipettors

SKU: H6505|Size: 100 Units/Pack

Plate Lids

SKU: LID-H1-10-N|Size: 100 Units/Case

PolyPestle™ Double Sided Pestle

SKU: C4010|Size: 50 Units/Pack

Power Supply for ProPette™

SKU: P6080-RC|Size: 1 Each

Power supply for Labeler

SKU: L9010-PS|Size: 1 Each

ProPette LE 12-Channel Pipette, 1 to 10 µL

SKU: P5212-10|Size: 1/Pack

ProPette LE 12-Channel Pipette, 2 to 20 µL

SKU: P5212-20|Size: 1/Pack

ProPette LE 12-Channel Pipette, 20 to 200 µL

SKU: P5212-200|Size: 1/Pack

ProPette LE 12-Channel Pipette, 30 to 300 µL

SKU: P5212-300|Size: 1/Pack

Recent Breakthroughs

Metabolomic insights into Lyme neuroborreliosis: Exploring cerebrospinal fluid for diagnostic clues.

Kuukkanen I, Pietikäinen A, Rissanen T, Hurme S, Kortela E, Hytönen J, Karonen M.

Ticks Tick Borne Dis

PMID:42001681

Free PMC article

Lyme neuroborreliosis (LNB), a disseminated manifestation of Lyme borreliosis (LB), arises when Borrelia burgdorferi sensu lato (Bbsl) spirochetes disseminate within the host and damage the peripheral nervous system and meninges, and in rare cases, also the parenchyma of the central nervous system (CNS). While early-stage LB is diagnosed clinically, accurate diagnosis of LNB requires cerebrospinal fluid (CSF) analysis, demonstrating pleocytosis and intrathecal synthesis of Bbsl-specific antibodies. There are, however, limitations in current LNB diagnostics, such as the unspecific nature of pleocytosis and post-treatment persistence of intrathecal antibodies necessitating the search for novel biomarkers. In this study, we employed untargeted ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) to profile small metabolites (<1500 Da) in CSF samples from subjects with definite acute LNB. Comparative analyses of metabolite profiles were performed between pretreatment subject samples (n = 63) and the following groups: (A) a subset of samples collected three weeks after treatment initiation from the same individuals (n = 36), (B) Bbsl antibody-negative subjects (non-LNB, n = 61), (C) subjects with other CNS infections (n = 21). Additionally, pretreatment LNB samples were compared between individuals with radiculitis (n = 40) and those without radiculitis (n = 23) (D). Out of 4222 molecular features (MFs) detected, 131 were prioritized based on statistical significance and magnitude of change for further detailed structural characterization. Altered metabolite classes included compounds from lysophospholipids [e.g., lysophosphatidylcholine (16:0), and lysophosphatidylethanolamine (18:0)], sphingomyelins [e.g., sphingomyelin (d18:1/14:0) and sphingomyelin d16:1/16:0)], sphingoid bases (e.g., d19:0 sphinganine, and 3-ketosphingosine), fatty acid amides (e.g., palmitoleamide and oleamide), cyclic phosphatidic acids [i.e., cyclic phosphatidic acid (16:0) and cyclic phosphatidic acid (18:2)], and amino acid metabolism (i.e., DL-glutamine, 5-hydroxytryptophan and DL-kynurenine). These findings underscore the potential of CSF metabolomics as a powerful complementary tool for diagnosing LNB and differentiating it from other CNS conditions. The identified metabolic signatures offer a foundation for future biomarker development and may enhance diagnostic precision, guide treatment strategies, and deepen our understanding of LNB pathogenesis.

Isotope Dilution NanoLC-MS/MS Quantitation of Methylglyoxal DNA-Protein Cross-Links: Formation and Repair in Human Cells.

Omondi RO, Barnes EM, Gurajala KC, Fisette G, Chaudray IA, Erber L.

Anal Chem

PMID:41995195

Free PMC article

DNA-protein cross-links (DPCs) represent a prevalent form of DNA damage that forms when cellular proteins become covalently trapped to DNA strands upon exposure to various endogenous and exogenous agents. Methylglyoxal is an endogenous metabolite that reacts with guanine and adenine bases in DNA and RNA, as well as cysteine, arginine, and lysine residues in proteins, generating advanced glycation end-products (AGEs), including DPCs. These modifications have been linked to human disease, including cancer, liver disease, diabetes, and neurodegenerative disorders. Herein, we present a mass spectrometry method for quantifying MGO-induced DNA-protein cross-links (DPCs) in human cells. We prepared an isotope 15 N 2 13 C 6 -dG-MGO-Lys internal standard and developed a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detecting and quantifying the formation and repair of dG-MGO-Lys DPCs in cells. Genomic DNA was extracted, subjected to sequential protease and nuclease digestion, purified by offline high-performance liquid chromatography (HPLC), and analyzed by LC-MS/MS. The method's standard curve showed a strong linear relationship across a concentration range of 10-1000 fmol ( R 2 = 0.9994). The method achieved limits of detection (LOD) and quantification (LOQ) of 10 and 20 fmol, respectively. Inhibition of proteasome and SPRTN activity revealed that SPRTN functions as a predominant proteolytic enzyme in MGO DPC repair. Overall, this analytical approach can offer valuable insights into the relevance of DPCs in diseases linked to elevated MGO levels.

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.

Rimmer MA, Wang J, Ranathunge TA, Shi Z, Li Y, Lee D, Chai SC, Lin W, Twarog NR, Ansari AZ, Shelat AA, Young BM, Chen T, Yang L.

Anal Chem

PMID:41997578

Free PMC article

Label-free high-throughput screening (HTS) technologies have greatly accelerated early drug discovery by allowing direct measurement of biochemical activity without the need for chemical labels. Techniques like MALDI-TOF, RapidFire, desorption electrospray ionization, and acoustic-mist ionization mass spectrometry have shown strong analytical performance. More recently, acoustic droplet ejection mass spectrometry (AEMS) has emerged as a next-generation platform capable of analyzing over a thousand data points per hour. In this study, we thoroughly evaluated an AEMS workflow using cytochrome P450 3A4 (CYP3A4) as a model enzyme. Screening a library of 9702 compounds, this work represents the first successful label-free HTS campaign for CYP3A4 inhibitors in a 384-well format using AEMS. The result using nifedipine as a substrate was comparable to the widely used P450-Glo luminescence assay but with fewer inhibitor hits identifications. To further compare and parse differences between the methods and the substrates, 819 hits were retested using AEMS with luciferin isopropyl acetal, the same substrate used in the luminescence assay. The findings confirmed that both the assay method and the substrate influence which inhibitors were detected. Overall, this study established AEMS as a robust and reliable tool for CYP3A4 inhibitor screening. It offers high analytical precision, fewer false positives, compatibility with multiple substrates, and direct detection of metabolites, providing a practical way to validate hits and improve confidence in early drug discovery efforts.

Quantification of the Active Metabolites 4-Methylaminoantipyrine and 4-Aminoantipyrine of Dipyrone From Human Plasma by LC-MS/MS.

Schmidt S, Ihmsen H, Golditz T, Schüttler J, Wehrfritz A.

Int J Anal Chem

PMID:42004449

Free PMC article

Dipyrone (metamizole) is widely used as a nonopioid analgesic in perioperative and intensive care settings; however, its administration may be associated with severe adverse effects. In this study, we describe an analytical assay for the quantification of the active metabolites 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA) in human plasma using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). In addition, the protein-unbound fractions of both metabolites were determined by ultrafiltration. Chromatographic separation was performed on a UPLC system using gradient elution, followed by MS/MS detection with an electrospray ionization source. The limits of detection for both metabolites were 100 ng/mL. Across the investigated concentration range (100-10,000 ng/mL), the relative error (%RE) ranged from -6.3% to +3.5%. Intra-day and inter-assay variability were below 10%. Method validation was conducted in accordance with the 2018 FDA Bioanalytical Method Validation Guidance, and all evaluated parameters met the required criteria for analytical accuracy and precision. The developed assay is suitable for monitoring dipyrone metabolites and may support the prevention of potential overdosing during prolonged analgesic therapy or in intensive care settings.

Metabolomics profiling of acute myelogenous leukemia patients to identify systemic differences associated with in vitro sensitivity to SYK inhibitors.

Brattås MK, Hatfield KJ, Paulsen Rye K, Reikvam H.

Metabolomics

PMID:41995902

Free PMC article

Introduction Acute myelogenous leukemia (AML) is a heterogeneous hematological malignancy. Despite therapeutic advances, high relapse rates and poor survival persist, underscoring the need for new treatment strategies. Spleen tyrosine kinase (SYK) is crucial for leukemic cell survival and proliferation, and SYK inhibition is being explored in AML therapy. Objective This study aimed to identify whether distinct baseline serum metabolic profiles can differentiate between AML patient samples exhibiting high versus low in vitro sensitivity to SYK inhibition. Methods Leukemic cells from 49 AML patients were evaluated for their in vitro antiproliferative response to SYK-selective inhibitors (entospletinib, RO9021) and dual SYK/FLT3 inhibitors (fostamatinib, TAK-659). Serum samples from the same patients were subjected to untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS). Bioinformatic analyses were applied to identify metabolic signatures associated with differential drug sensitivity. Results Hierarchical clustering identified three drug sensitivity groups, i.e. high, intermediate, low. Untargeted metabolomics identified 1204 metabolites across major pathways. Comparative analyses revealed distinct metabolic differences between patients with high versus low sensitivity to SYK inhibitors. For dual SYK/FLT3 inhibitors, lipid metabolites consistently were more discriminative for drug response, while for SYK selective inhibitors the profiles were more heterogeneous. Pathway analyses highlighted alterations in amino acid, lipid, and xenobiotic metabolism, including arginine biosynthesis and glycine/serine metabolism, as potential determinators for effect of SYK inhibition. Conclusion This study highlights potential metabolite signatures associated with in vitro SYK-targeted therapy in AML. These findings support future research to identify potential serum biomarkers, guiding personalized SYK inhibition strategies in AML.