Terminal Deoxynucleotidyl Transferase (TdT) (FITC)
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Catalog numberGEN603660
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Price:
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Size0.025 miligrams
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Also known asTerminal Deoxynucleotidyl Transferase
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Other namesterminal deoxynucleotidyl transferase; N/A; terminal deoxynucleotidyl transferase; N/A; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nametdt; N/A; N/A; N/A
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Gene name synonimstdt; N/A; N/A; N/A
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Other gene namestdt; N/A; N/A; N/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1/IgG2a
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Clone7E150, 7E151
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Host organismMouse (Mus musculus)
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Source organismHuman (Homo sapiens)
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Species reactivityHuman (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityRecognizes human terminal deoxynucleotidyl transferase (TdT) (mixture of 3 monoclonal antibodies).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodPurified immunoglobulin
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Form AppearanceSupplied as a liquid in PBS, 0.09% sodium azide, 1% BSA.
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ConcentrationN/A
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Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applicationsFlow Cytometry (FC/FACS)
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameTerminal Deoxynucleotidyl Transferase (TdT) (FITC)
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative nameAnti-Terminal Deoxynucleotidyl Transferase (TdT) (FITC)
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data