Anti-Mouse NK Cells (NK1.1), FITC (Clone PK136) (mouse IgG2a)
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Catalog numberGEN520385
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Price:
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Size100ug
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Also known asNK Cells (NK1.1)
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Other namesN/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameN/A
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeMouse IGg2a
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ClonePK136
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Host organismMouse (Mus musculus)
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityNK Cells (NK1.1, CD161) (Mouse NK Cells (NK1.1)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC
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Concentration0.1 miligram per 1 mililiter
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Storage and shippingFor all formats, store the antibody at +4 degrees Celsius. DO NOT FREEZE PE and APC conjugates. For long term storage (Purified, Biotin and FITC), aliquot and freeze at -20C. The unused portion of the antibody should be stored at -20 degrees Celsius in volumes suited for single use. Avoid freeze/defrost cycles.
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Tested applicationsImmunohistochemistry (IHC)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolCD160
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Short nameAnti-Mouse NK Cells (NK1 1), FITC (Clone PK136) (mouse IgG2a)
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Techniqueanti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Hostmouse
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IsotypeIgG2a
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameAnti-Anti-Mouse NK Cells (NK1.1), FITC (Clone PK136) (mouse IgG2a)
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Alternative techniquemurine, antibodies, fluorescine
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Clone namePK136
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Tissuecells
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Gene info
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Identity
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Gene
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Long gene nameCD160 molecule
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Synonyms gene name
- CD160 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data