Rabbit Polyclonal antibody to detect Catalase
Catalase, Human Erythrocytes (Cat. No. 4712)
Affinity purified antibody is supplied in liquid form in PBS pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
Antibody's positive controles
Western blot: Jurkat, 3T3, HepG2 cell lysates, human catalase
Approved applications with antibody dilution
Western blot: 1-4 µg
Catalase protein occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Catalase is a key antioxidant enzyme in the body’s defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Shipped to your lab under gel pack
Manifactured in USA. For Research Use Only! Not to be used in humans
This antibody is available to order since May 2017
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.